Welcome to IVF
Welcome to IVF
Welcome to IVF
Prenatal Diagnosis
This involves genetic analysis of cells of fetal origin in various disorders by chorionic villus sampling, amniocentesis, or cord blood sampling, by karyotyping and FISH.
Indications:
  • Women with advanced maternal age
  • Previous child with a chromosome abnormality. 
  • Women who have had previous still births or early neonatal deaths 
  • Parents with balanced translocations 
  • Cases in which the Triple Marker test of alpha fetoprotein, serum oestriol and beta HCG shows a high risk 
  • Abnormalities seen on ultrasonography
The FISH technique is very useful for rapidly detecting aneuploidies in high-risk pregnancies. A ‘high risk’ for Trisomy 21 by the Double marker / Triple test or Ultrasonography causes a lot of anxiety to the expectant couple, which can be relieved in a day by FISH on uncultured amniocytes. Karyotyping is simultaneously done to rule out other structural and numerical chromosome abnormalities. If the quantity of chorionic villi obtained in a biopsy is insufficient for karyotyping, FISH is still possible from interphase nuclei.
PRENATAL DIAGNOSIS
Sample:
  • Amniotic fluid (8 ml for FISH; 15 ml for karyotyping; in 2-4 plain – red top – vaccutainers). In case of a sanguineous tap, collect in heparin vaccutainers.
  • Cord blood (1ml in sodium heparin – green top – vaccutainer). 
  • CVS in sterile normal saline with 2 drops of Gentamycin and sodium heparin or in media tubes supplied by the laboratory.