Welcome to IVF
Welcome to IVF
Welcome to IVF
FISH
Fluorescence in situ hybridization (FISH) is a new and rapid procedure to detect certain common chromosome abnormalities. We mainly use directly labeled commercially available Vysis (Abbott) FISH probes which are complementary to the region of interest on a particular chromosome. The analysis is usually done by counting colored dots under a fluorescent microscope. The cells are pretreated and fixed on slides. The slides are dehydrated, co-denatured with appropriate probe, hybridized, washed and mounted in antifade solution together with counterstain. The FISH signals are observed under a Zeiss fluorescence microscope using appropriate filters and the images are captured and analyzed with the Metasystems software. FISH is a rapid technique to detect common aneuploidies and low-grade mosaicism mainly in prenatal diagnosis, products of conception and pediatric cases. Common translocations in leukemia can also be analysed semi-quantitatively, to determine response to therapy and detect minimal residual disease. Analysis by FISH is possible even on interphase nuclei.